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OriGene ilk human cdna orf
Ilk Human Cdna Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ilk+human+cdna+orf/Integrin+Linked+Kinase+(ILK)+(NM_004517)+Human+Tagged+ORF+Clone/pm28138714-54-5-15
Average 90 stars, based on 1 article reviews

ilk human cdna orf - by Bioz Stars, 2026-10

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Article Title: Overexpression of ILK promotes temozolomide resistance in glioma cells.
Article Snippet: The commercial vector is the ILK Human cDNA ORF clone (NM_004517), which was purchased from OriGene Technologies, Inc. (Rockville, MD, USA; cat. no. RC20154L1).

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TG2 expression activates ILK in OC cells. A , WB for p-ILK Ser246 , ILK, TG2, and GAPDH in SKOV3 cells stably transduced with scrambled shRNA (sh-Ctr) or TG2-targeting shRNA (sh-TG2) and plated on FN-coated plates for 2 h. Densitometry quantifies TG2 expression levels and p-ILK Ser246 /ILK ratio (N = 3; ∗∗∗ p < 0.01, ∗∗∗∗ p < 0.0001). B , IF staining for TG2 (Alexa Fluor 488, green ) and p-ILK Ser246 (Alexa Fluor 568, red ) in SKOV3 cells sh-Ctr or sh-TG2 KD and plated on FN-coated plates for 2 h. Protein colocalization is identified by yellow spectra on merged images. C , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). D , quantification of colocalized proteins was calculated by volume area of green over red spectra in sh-Ctr (N = 3; Pearson’s rank correlation = 0.42) versus sh-TG2 SKOV3 cells (N = 3; Pearson’s rank correlation = 0.006; p < 0.0001). E , TG2–p-ILK Ser246 colocalization ( red dots ) and phalloidin ( green ) detected by PLA in four primary human cells isolated from deidentified malignant ascites fluid specimens and plated on plastic or FN-coated plates for 2 h. Representative images are shown (magnification, 200×). Bar represents 20 μm. F , quantification of the number of total TG2–p-ILK Ser246 red dots per sample in a diagram (N = 3; ∗ p < 0.05, ∗∗ p < 0.01). G , correlation between TGM2 and ILK m RNA expression levels (Spearman r = 0.15, p = 1.2e-3 and Pearson r = 0.15, p = 2.9e-4) in the TCGA ovarian cancer database obtained from cBioPortal. H , overall survival curves generated using the Kaplan–Meier plot for tumors expressing higher than median levels of TGM2 and of ILK versus those expressing lower than median levels of TGM2 and of ILK in HGSOC tumor microarray data of 14 datasets from seven different array platforms using OvMark (HR = 1.52 and p = 0.02221). FN, fibronectin; HGSOC, high-grade serous ovarian cancer; HR, hazard ratio; IF, immunofluorescence; ILK, integrin-linked kinase; OC, ovarian cancer; PLA, proximity ligation assay; TCGA, The Cancer Genome Atlas; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: TG2 expression activates ILK in OC cells. A , WB for p-ILK Ser246 , ILK, TG2, and GAPDH in SKOV3 cells stably transduced with scrambled shRNA (sh-Ctr) or TG2-targeting shRNA (sh-TG2) and plated on FN-coated plates for 2 h. Densitometry quantifies TG2 expression levels and p-ILK Ser246 /ILK ratio (N = 3; ∗∗∗ p < 0.01, ∗∗∗∗ p < 0.0001). B , IF staining for TG2 (Alexa Fluor 488, green ) and p-ILK Ser246 (Alexa Fluor 568, red ) in SKOV3 cells sh-Ctr or sh-TG2 KD and plated on FN-coated plates for 2 h. Protein colocalization is identified by yellow spectra on merged images. C , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). D , quantification of colocalized proteins was calculated by volume area of green over red spectra in sh-Ctr (N = 3; Pearson’s rank correlation = 0.42) versus sh-TG2 SKOV3 cells (N = 3; Pearson’s rank correlation = 0.006; p < 0.0001). E , TG2–p-ILK Ser246 colocalization ( red dots ) and phalloidin ( green ) detected by PLA in four primary human cells isolated from deidentified malignant ascites fluid specimens and plated on plastic or FN-coated plates for 2 h. Representative images are shown (magnification, 200×). Bar represents 20 μm. F , quantification of the number of total TG2–p-ILK Ser246 red dots per sample in a diagram (N = 3; ∗ p < 0.05, ∗∗ p < 0.01). G , correlation between TGM2 and ILK m RNA expression levels (Spearman r = 0.15, p = 1.2e-3 and Pearson r = 0.15, p = 2.9e-4) in the TCGA ovarian cancer database obtained from cBioPortal. H , overall survival curves generated using the Kaplan–Meier plot for tumors expressing higher than median levels of TGM2 and of ILK versus those expressing lower than median levels of TGM2 and of ILK in HGSOC tumor microarray data of 14 datasets from seven different array platforms using OvMark (HR = 1.52 and p = 0.02221). FN, fibronectin; HGSOC, high-grade serous ovarian cancer; HR, hazard ratio; IF, immunofluorescence; ILK, integrin-linked kinase; OC, ovarian cancer; PLA, proximity ligation assay; TCGA, The Cancer Genome Atlas; TG2, tissue transglutaminase; WB, Western blot.

Article Snippet: Full-length clone DNA of human TGM2 with C-terminal His tag and full-length clone DNA of human ILK with C-terminal FLAG tag were inserted into pCMV3 vectors (SinoBiological) and stably transfected in sh-TG2 SKOV3 (#1) and OVCAR-5 (#1) cells using DreamFect Gold transfection reagent, followed by hygromycin selection.

Techniques: Expressing, Stable Transfection, Transduction, shRNA, Staining, Software, Isolation, RNA Expression, Generated, Microarray, Immunofluorescence, Proximity Ligation Assay, Western Blot

TG2 interacts with ILK and activated ILK in OC cells. A , equal amounts of lysates of SKOV3 cells were immunoprecipitated with TG2 and ILK antibodies (Abs). WB was performed using TG2, ILK, and GAPDH Abs. B , co-IP with anti-TG2 and anti-ILK Abs and WB for TG2 and ILK using full-length recombinant TG2 and ILK. C , His-tagged TG2 and FLAG-tagged ILK were immunoprecipitated from lysates of sh-TG2 SKOV3 cells stably cotransfected with pCMV3- TGM2 -C-His and pCMV3- ILK -C-FLAG expression plasmids. WB was performed using His, FLAG, and GAPDH Abs. Nonspecific IgG was used as control. D and G , IF staining for TG2 (Alexa Fluor 488, green ), ILK ( D ), and p-ILK Ser246 ( G ) (Alexa Fluor 568, red ) in SKOV3 cells sh-TG2 KD stably cotransfected with pCMV3- TGM2 -C-His and plated on FN-coated plates for 2 h. Protein colocalization is identified by yellow spectra on merged images. E and H , quantification of Alexa Fluor 568 ( red ) ILK ( E ) and p-ILK Ser246 ( H ) in permeabilized versus nonpermeabilized cells was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). F and I , quantification of colocalized proteins was calculated by volume area of green over red spectra in permeabilized (N = 3; Pearson’s rank correlation = 0.715) ( F ) and (N = 3; Pearson’s rank correlation = 0.76) ( I ) versus nonpermeabilized sh-TG2 SKOV3 cells stably cotransfected with pCMV3- TGM2 -C-His (N = 3; Pearson’s rank correlation = 0.007; p < 0.0001) ( F ) and (N = 3; Pearson’s rank correlation = 0.005; p < 0.0001) ( I ). co-IP, coimmunoprecipitation; FN, fibronectin; IF, immunofluorescence; IgG, immunoglobulin G; ILK, integrin-linked kinase; KD, knockdown; OC, ovarian cancer; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: TG2 interacts with ILK and activated ILK in OC cells. A , equal amounts of lysates of SKOV3 cells were immunoprecipitated with TG2 and ILK antibodies (Abs). WB was performed using TG2, ILK, and GAPDH Abs. B , co-IP with anti-TG2 and anti-ILK Abs and WB for TG2 and ILK using full-length recombinant TG2 and ILK. C , His-tagged TG2 and FLAG-tagged ILK were immunoprecipitated from lysates of sh-TG2 SKOV3 cells stably cotransfected with pCMV3- TGM2 -C-His and pCMV3- ILK -C-FLAG expression plasmids. WB was performed using His, FLAG, and GAPDH Abs. Nonspecific IgG was used as control. D and G , IF staining for TG2 (Alexa Fluor 488, green ), ILK ( D ), and p-ILK Ser246 ( G ) (Alexa Fluor 568, red ) in SKOV3 cells sh-TG2 KD stably cotransfected with pCMV3- TGM2 -C-His and plated on FN-coated plates for 2 h. Protein colocalization is identified by yellow spectra on merged images. E and H , quantification of Alexa Fluor 568 ( red ) ILK ( E ) and p-ILK Ser246 ( H ) in permeabilized versus nonpermeabilized cells was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). F and I , quantification of colocalized proteins was calculated by volume area of green over red spectra in permeabilized (N = 3; Pearson’s rank correlation = 0.715) ( F ) and (N = 3; Pearson’s rank correlation = 0.76) ( I ) versus nonpermeabilized sh-TG2 SKOV3 cells stably cotransfected with pCMV3- TGM2 -C-His (N = 3; Pearson’s rank correlation = 0.007; p < 0.0001) ( F ) and (N = 3; Pearson’s rank correlation = 0.005; p < 0.0001) ( I ). co-IP, coimmunoprecipitation; FN, fibronectin; IF, immunofluorescence; IgG, immunoglobulin G; ILK, integrin-linked kinase; KD, knockdown; OC, ovarian cancer; TG2, tissue transglutaminase; WB, Western blot.

Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Recombinant, Stable Transfection, Expressing, Staining, Software, Immunofluorescence, Western Blot

TG2–FN clusters activate β-catenin in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: TG2–FN clusters activate β-catenin in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Techniques: Stable Transfection, Transduction, shRNA, Expressing, Transfection, Staining, Software, Luciferase, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Binding Assay, Western Blot

Functional TG2 and integrin β1 inhibition disrupts β-catenin signaling in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, and GAPDH in OVCAR-5 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) or integrin β1 (clone P5D2) and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios (N = 3; ∗ p < 0.05; ∗∗ p < 0.01). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). C , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A plated on FN for 2 h. D , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). E , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid, treated with 4G3 or P5D2 Abs and/or Wnt-3A, and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). F , real-time PCR for c-Myc in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h (N = 6; ∗∗∗∗ p < 0.0001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: Functional TG2 and integrin β1 inhibition disrupts β-catenin signaling in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, and GAPDH in OVCAR-5 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) or integrin β1 (clone P5D2) and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios (N = 3; ∗ p < 0.05; ∗∗ p < 0.01). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). C , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A plated on FN for 2 h. D , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). E , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid, treated with 4G3 or P5D2 Abs and/or Wnt-3A, and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). F , real-time PCR for c-Myc in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h (N = 6; ∗∗∗∗ p < 0.0001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Techniques: Functional Assay, Inhibition, Binding Assay, Expressing, Staining, Software, Luciferase, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot

ILK functional inhibition results in OC cells' proliferation and migration inhibition. A , CCK-8 assay quantifies proliferation of SKOV3 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) and/or Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). B , scratch assay quantifies migration of SKOV3 cells treated with 4G3 and/or Wnt-3A (N = 8; ∗∗ p < 0.01; ∗∗∗∗ p < 0.0001). C , CCK-8 assay quantifies proliferation of SKOV3 cells treated with cpd-22 and/or Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). D , scratch assay quantifies migration of SKOV3 cells treated with cpd-22 and/or Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). E , CCK-8 assay quantifies proliferation of ILK-KD versus sh-Ctr SKOV3 cells treated with or not with Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). F , scratch assay quantifies migration of ILK-KD versus sh-Ctr SKOV3 cells treated with or not with Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). G , overall survival curves generated using the Kaplan–Meier plot for tumors expressing higher than median levels of TGM2 , ITGB1 , FN1 , and of ILK versus those expressing lower than median levels of TGM2 , ITGB1 , FN1 , and of ILK in HGSOC tumor microarray (HR = 1.4, p = 0.0046). H , proposed mechanistic model. CCK-8, Cell Counting Kit 8; cpd-22, compound 22; FN, fibronectin; HGSOC, high-grade serous ovarian cancer; HR, hazard ratio; ILK, integrin-linked kinase; KD, knockdown; OC, ovarian cancer; TG2, tissue transglutaminase.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: ILK functional inhibition results in OC cells' proliferation and migration inhibition. A , CCK-8 assay quantifies proliferation of SKOV3 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) and/or Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). B , scratch assay quantifies migration of SKOV3 cells treated with 4G3 and/or Wnt-3A (N = 8; ∗∗ p < 0.01; ∗∗∗∗ p < 0.0001). C , CCK-8 assay quantifies proliferation of SKOV3 cells treated with cpd-22 and/or Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). D , scratch assay quantifies migration of SKOV3 cells treated with cpd-22 and/or Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). E , CCK-8 assay quantifies proliferation of ILK-KD versus sh-Ctr SKOV3 cells treated with or not with Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). F , scratch assay quantifies migration of ILK-KD versus sh-Ctr SKOV3 cells treated with or not with Wnt-3A (N = 8; ∗∗∗∗ p < 0.0001). G , overall survival curves generated using the Kaplan–Meier plot for tumors expressing higher than median levels of TGM2 , ITGB1 , FN1 , and of ILK versus those expressing lower than median levels of TGM2 , ITGB1 , FN1 , and of ILK in HGSOC tumor microarray (HR = 1.4, p = 0.0046). H , proposed mechanistic model. CCK-8, Cell Counting Kit 8; cpd-22, compound 22; FN, fibronectin; HGSOC, high-grade serous ovarian cancer; HR, hazard ratio; ILK, integrin-linked kinase; KD, knockdown; OC, ovarian cancer; TG2, tissue transglutaminase.

Techniques: Functional Assay, Inhibition, Migration, CCK-8 Assay, Binding Assay, Wound Healing Assay, Generated, Expressing, Microarray, Cell Counting

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